Microbial dynamics during aerobic exposure of corn silage stored under oxygen barrier or polyethylene films.
Articolo
Data di Pubblicazione:
2011
Abstract:
The aims of this study were to compare the effects of sealing forage corn with a new oxygen barrier film with
those obtained by using a conventional polyethylene film. This comparison was made during both ensilage and
subsequent exposure of silage to air and included chemical, microbiological, and molecular (DNA and RNA)
assessments. The forage was inoculated with a mixture of Lactobacillus buchneri, Lactobacillus plantarum, and
Enterococcus faecium and ensiled in polyethylene (PE) and oxygen barrier (OB) plastic bags. The oxygen
permeability of the PE and OB films was 1,480 and 70 cm3 m2 per 24 h at 23°C, respectively. The silages were
sampled after 110 days of ensilage and after 2, 5, 7, 9, and 14 days of air exposure and analyzed for
fermentation characteristics, conventional microbial enumeration, and bacterial and fungal community fingerprinting
via PCR-denaturing gradient gel electrophoresis (DGGE) and reverse transcription (RT)-PCRDGGE.
The yeast counts in the PE and OB silages were 3.12 and 1.17 log10 CFU g1, respectively, with
corresponding aerobic stabilities of 65 and 152 h. Acetobacter pasteurianus was present at both the DNA and
RNA levels in the PE silage samples after 2 days of air exposure, whereas it was found only after 7 days in the
OB silages. RT-PCR-DGGE revealed the activity of Aspergillus fumigatus in the PE samples from the day 7 of
air exposure, whereas it appeared only after 14 days in the OB silages. It has been shown that the use of an
oxygen barrier film can ensure a longer shelf life of silage after aerobic exposure.
Tipologia CRIS:
03A-Articolo su Rivista
Elenco autori:
P. Dolci; E. Tabacco; L. Cocolin; G. Borreani
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