Aneuploidy and genome organization in sperm nuclei of cattle and river buffalo detected by fluorescence in situ hybridization (FISH) and chromosome microdissection
Articolo
Data di Pubblicazione:
2005
Abstract:
New technologies in molecular cytogenetics have remarkably facilitated the visualization
of whole chromosomes or subchromosomal regions in interphase nuclei. Consequently, both somatic
and sperm nuclei have been under investigation, in order to study the territorial organization of genetically
‘active’ versus ‘inactive’ genomes. Purpose of the present study was to investigate whether in river buffalo
sperm nuclei, chromosomes X-, Y- and 5p (corresponding to chromosome 29 in cattle) maintain or change
their relative position compared to cattle. Furthermore, since chromosome organization in the nucleus could
affect meiotic segregation, we decided to study X-Y chromosome segregation in river buffalo sperm and
developed a dual colour FISH method for evaluating the baseline level of aneuploidy in spermatozoa of this
species, that could be used as reference for further investigations on the genotoxic effects of environmental
mutagens and bio-hazards directly on the germ-cell line.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
chromosome; aneuploidy; sperm architecture
Elenco autori:
DI BERARDINO D.; NICODEMO D.; COPPOLA G.; PAUCIULLO A.; COSENZA G.; RAMUNNO L.; COLETTA A.; RUBES J.
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