Data di Pubblicazione:
2015
Abstract:
-Plant-invading microbes betray their presence to a plant by exposure of antigenic molecules
such as small, secreted proteins called ‘effectors’. In Fusarium oxysporum f. sp. lycopersici
(Fol ) we identified a pair of effector gene candidates, AVR2-SIX5, whose expression is
controlled by a shared promoter.
-The pathogenicity of AVR2 and SIX5 Fol knockouts was assessed on susceptible and resis-
tant tomato (Solanum lycopersicum) plants carrying I-2. The I-2 NB-LRR protein confers resis-
tance to Fol races carrying AVR2.
- Like Avr2, Six5 was found to be required for full virulence on susceptible plants. Unexpect-
edly, each knockout could breach I-2-mediated disease resistance. So whereas Avr2 is suffi-
cient to induce I-2-mediated cell death, Avr2 and Six5 are both required for resistance. Avr2
and Six5 interact in yeast two-hybrid assays as well as in planta. Six5 and Avr2 accumulate in
xylem sap of plants infected with the reciprocal knockouts, showing that lack of I-2 activation
is not due to a lack of Avr2 accumulation in the SIX5 mutant.
-The effector repertoire of a pathogen determines its host specificity and its ability to manip-
ulate plant immunity. Our findings challenge an oversimplified interpretation of the gene-for-
gene model by showing requirement of two fungal genes for immunity conferred by one
resistance gene.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Avirulence; Disease resistance; Effector proteins; Fusarium oxysporum; Gene-for-gene; I-2; Tomato (Solanum lycopersicum); Plant Science; Physiology
Elenco autori:
Ma, Lisong; Houterman, Petra M.; Gawehns, Fleur; Cao, Lingxue; Sillo, Fabiano; Richter, Hanna; Clavijo-Ortiz, Myriam J.; Schmidt, Sarah M.; Boeren, Sjef; Vervoort, Jacques; Cornelissen, Ben J.C.; Rep, Martijn; Takken, Frank L.W.
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