PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
Articolo
Data di Pubblicazione:
2016
Abstract:
Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. However, mechanisms coordinating α5β1 integrin-mediated extracellular FN endocytosis and exocytosis of newly synthesized FN remain elusive. Here we show that, on Rab21-elicited internalization, FN-bound/active α5β1 is recycled to the EC surface. We identify a pathway, comprising the regulators of post-Golgi carrier formation PI4KB and AP-1A, the small GTPase Rab11B, the surface tyrosine phosphatase receptor PTPRF and its adaptor PPFIA1, which we propose acts as a funnel combining FN secretion and recycling of active α5β1 integrin from the trans-Golgi network (TGN) to the EC surface, thus allowing FN fibrillogenesis. In this framework, PPFIA1 interacts with active α5β1 integrin and localizes close to EC adhesions where post-Golgi carriers are targeted. We show that PPFIA1 is required for FN polymerization-dependent vascular morphogenesis, both in vitro and in the developing zebrafish embryo.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Integrins; Angiogenesis; Endothelial Cells; Endocytosis; Trans-Golgi network; Fibronectin; Embryonic development; Zebrafish
Elenco autori:
Mana, Giulia; Clapero, Fabiana; Panieri, Emiliano; Panero, Valentina; Böttcher, Ralph T; Tseng, Hui-Yuan; Saltarin, Federico; Astanina, Elena; Wolanska, Katarzyna I; Morgan, Mark R; Humphries, Martin J; Santoro, Massimo M; Serini, Guido; Valdembri, Donatella
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