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  1. Pubblicazioni

Focusing on the goat casein complex

Articolo
Data di Pubblicazione:
2006
Abstract:
The analysis of casein polymorphisms in goat species is rather difficult, because of a large number of muta- tions at each locus, and the tight linkage involving the 4 casein genes. Three goat breeds from Northern Italy, Orobica, Verzasca, and Frisa, were analyzed at the ca- sein complex by milk isoelectrofocusing and analyses at the DNA level to identify the majority of all known polymorphisms. The casein gene structure of the 3 local breeds at αS1-casein (CSN1S1), β-casein (CSN2), αS2- casein (CSN1S2), and κ-casein (CSN3) was compared with that of Camosciata, a more widely distributed breed. A new allele was identified and characterized at CSN2 gene, which seemed to be specific to the Frisa breed. It was named CSN2*E, and was characterized by a transversion TCT→TAT responsible for the amino acid exchange Ser166→Tyr166 in the mature protein. The casein haplotype structure is highly different among breeds. A total of 26 haplotypes showed a frequency higher than 0.01 in at least 1 of the 4 breeds considered, with 12, 3, 5, and 19 haplotypes in Frisa, Orobica, Verzasca, and Camosciata breeds, respectively. Only 13 haplotypes occurred at a frequency higher than 0.05 in at least 1 breed. With the molecular knowledge of each locus, the ancestral haplotype coding for CSN1S1*B, CSN2*A, CSN1S2*A, and CSN3*B protein variants can be postulated. A protein evolutionary model consid- ering the whole casein haplotype is proposed.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
casein genotype, goat, proteome, variability
Elenco autori:
Caroli A., Chiatti F., Chessa S., Rignanese D., Bolla P., Pagnacco G.
Autori di Ateneo:
CHESSA Stefania
Link alla scheda completa:
https://iris.unito.it/handle/2318/1688942
Pubblicato in:
JOURNAL OF DAIRY SCIENCE
Journal
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-33746908160&doi=10.3168%2fjds.S0022-0302%2806%2972592-9&partnerID=40&md5=46269b6c832927562d5020a6d84586c3
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