Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes
Articolo
Data di Pubblicazione:
2018
Abstract:
The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin- and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Animals; Astrocytes; Calcium; Interneurons; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neocortex; Neuronal Plasticity; Optogenetics; Parvalbumins; Patch-Clamp Techniques; Receptors, GABA-B; Somatosensory Cortex; Somatostatin; Signal Transduction; Chemistry (all); Biochemistry, Genetics and Molecular Biology (all); Physics and Astronomy (all)
Elenco autori:
Mariotti, Letizia; Losi, Gabriele; Lia, Annamaria; Melone, Marcello; Chiavegato, Angela; Gómez-Gonzalo, Marta; Sessolo, Michele; Bovetti, Serena; Forli, Angelo; Zonta, Micaela; Requie, Linda Maria; Marcon, Iacopo; Pugliese, Arianna; Viollet, Cécile; Bettler, Bernhard; Fellin, Tommaso; Conti, Fiorenzo; Carmignoto, Giorgio
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