Loss of bhlha9 impairs thermotaxis and formalin-evoked pain in a sexually dimorphic manner.
Articolo
Data di Pubblicazione:
2020
Abstract:
C-LTMRs are known to convey affective aspects of touch and to modulate injury-induced pain in humans and mice. However, a role for these neurons in temperature sensation has been suggested, but not fully demonstrated. Here, we report that deletion of C-low-threshold mechanoreceptor (C-LTMR)- expressed bhlha9 causes impaired thermotaxis behavior and exacerbated formalin-evoked pain in male, but not female, mice. Positive modulators of GABAA receptors failed to relieve inflammatory formalin pain and failed to decrease the frequency of spontaneous excitatory post-synaptic currents (sEPSCs) selectively in bhlha9 knockout (KO) males. This could be explained by a drastic change in the GABA content of lamina II inner inhibitory interneurons contacting C-LTMR central terminals. Finally, C-LTMR-specific deep RNA sequencing revealed more genes differentially expressed in male than in female bhlha9 KO C-LTMRs. Our data consolidate the role of C-LTMRs in modulation of formalin pain and provide in vivo evidence of their role in the discriminative aspects of temperature sensation.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
bhlha9, mice, thermotaxis, formalin-evoked pain, C-LTMRs
Elenco autori:
Bohic Manon, Marics Irene, Santos Catarina, Malapert Pascale, Ben-Arie Nissim, Salio Chiara, Reynders Ana, Le Feuvre Yves, Saurin Andrew J, Moqrich Aziz
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