Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution
Articolo
Data di Pubblicazione:
2019
Abstract:
Conventional human embryonic stem cells are considered to be primed pluripotent but can be induced to enter a naive state. However, the transcriptional features associated with naive and primed pluripotency are still not fully understood. Here we used single-cell RNA sequencing to characterize the differences between these conditions. We observed that both naive and primed populations were mostly homogeneous with no clear lineage-related structure and identified an intermediate subpopulation of naive cells with primed-like expression. We found that the naive-primed pluripotency axis is preserved across species, although the timing of the transition to a primed state is species specific. We also identified markers for distinguishing human naive and primed pluripotency as well as strong co-regulatory relationships between lineage markers and epigenetic regulators that were exclusive to naive cells. Our data provide valuable insights into the transcriptional landscape of human pluripotency at a cellular and genome-wide resolution.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
early embryonic development; evolution; heterogeneity; human embryonic stem cells; naive; pluripotency; primed; single-cell RNA-seq; Cell Line; Human Embryonic Stem Cells; Humans; Epigenesis, Genetic; Sequence Analysis, RNA; Single-Cell Analysis; Transcription, Genetic
Elenco autori:
Messmer T.; von Meyenn F.; Savino A.; Santos F.; Mohammed H.; Lun A.T.L.; Marioni J.C.; Reik W.
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