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  1. Pubblicazioni

Milk fat miRNome changes in response to LPS challenge in Holstein cows

Articolo
Data di Pubblicazione:
2023
Abstract:
Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (miRNome) of milk fat, using microarray analyses in cows. Cows were fed a lactation diet at ad libitum intake (n = 6). At 27 ± 3 days in milk, cows were injected with 50 µg of LPS Escherichia coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS−) and 6.5 h after LPS challenge (LPS +) from the same cows. Microarray analysis was performed using customized 8 × 60 K ruminant miRNA microarrays to compare LPS− to LPS + miRNome. In silico functional analyses were performed using OmicsNet and Mienturnet software. MiRNome comparison between LPS− and LPS + identified 37 differentially abundant miRNAs (q-value ≤ 0.05). The predicted target genes of the 37 differentially abundant miRNAs are mostly involved in cell life including apoptosis, cell cycle, proliferation and differentiation and in gene expression processes. MiRNome analyses suggest that miRNAs profile is related to the inflammation response of the mammary gland. In conclusion, we demonstrated that milk fat might be an easy and rapid source of miRNAs that are potential indicators of early mastitis in cows.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
miRNomes, milk fat, dairy cow, inflammation, LPS
Elenco autori:
Christine Leroux, Matteo Cuccato, Karol Pawłowski, Francesca Tiziana Cannizzo, Paola Sacchi, José A. A. Pires, Yannick Faulconnier
Autori di Ateneo:
CANNIZZO Francesca Tiziana
CUCCATO Matteo
SACCHI Paola
Link alla scheda completa:
https://iris.unito.it/handle/2318/1944402
Link al Full Text:
https://iris.unito.it/retrieve/handle/2318/1944402/1211017/Leroux%20et%20al%202023.pdf
Pubblicato in:
VETERINARY RESEARCH
Journal
  • Dati Generali
  • Aree Di Ricerca

Dati Generali

URL

https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-023-01231-4

Aree Di Ricerca

Settori (12)


LS9_10 - Veterinary and applied animal sciences - (2022)

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