Data di Pubblicazione:
2015
Abstract:
Rapid and sensitive competitive enzymatic immunoassays for measuring most relevant aflatoxins in eggs
have been developed by synthesizing two hapten derivatives. Polyclonal antibodies raised against a
hapten obtained from aflatoxin B1 (AFB1) were exploited to set an AFB1-selective assay, whereas antibodies
obtained through immunising with a hapten derived from aflatoxin M1 (AFM1) allowed us to
detect four principal aflatoxins (B1, G1, B2, and G2) and the most relevant AFB1 metabolite (AFM1) with
detection limits in eggs of 0.3 mg kg-1 for AFB1, AFG1, and AFM1 and 3 mg kg-1 for AFB2 and AFG2,
respectively. We also established a rapid and simple protocol for extracting aflatoxins from eggs by
employing aqueous methanol (70%) followed by partitioning with hexane to remove fats. The whole
analytical process is simple, very rapid (the extraction requires 14 min, and the assay is completed in
30 min) and proved to be accurate and precise enough (recoveries ranged from 84 to 100% and RSD%
were within 20% for intra- and inter-assay experiments) to be proposed as a first level screening method
for the monitoring of the occurrence of aflatoxins in egg
Tipologia CRIS:
03A-Articolo su Rivista
Elenco autori:
Anfossi, Laura; Di Nardo, Fabio; Giovannoli, Cristina; Passini, Cinzia; Baggiani, Claudio
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