ESTROGEN DERIVATIVES OF TRANSITION-METAL CARBONYL CLUSTERS FOR ANALYTICAL DETECTION ENHANCEMENT

We herein describe the labelling of ethynylestradiol with metal carbonyl fragments, namely -Co2(CO)6, -M3(mu-CO)(CO)9 (M = Ru, Os) and -Ru3(mu-H)(CO)9. The solution structure of RU3(mu-CO)(CO)9 (ethynylestradiol) cluster has been studied by means of variable-temperature (VT) C-13 NMR spectroscopy. This derivatization can be useful for detection enhancement in high-performance liquid chromatography (HPLC) separation, since the metal carbonyl moieties act as efficient markers for the UV detection mode at the usually employed 254 nm wavelength.