Evidence that the Human Cytomegalovirus 46 kDa UL72 protein is not an active dUTPase but a late protein dispensable for replication in fibroblasts
Articolo
Data di Pubblicazione:
2004
Abstract:
The Human Cytomegalovirus (HCMV) UL72 gene is considered to be the equivalent of the dUTPase gene of the Alpha- and Gammaherpesviruses.
To characterize its function, the expression profiles of UL72 at both the RNA and the protein level were determined. The gene
is expressed with a late kinetics and the corresponding UL72 46-kDa protein accumulates late during infection in the cytoplasm of infected
cells. The pUL72 was expressed in E. coli and the purified recombinant protein did not display a detectable dUTPase activity. The viral yields
of reconstituted HCMV RVDUL72 viruses carrying a deletion within the UL72 ORF demonstrated a moderate growth defect following low
MOI infections, whereas their DNA synthesis profiles were not significantly different from those of the parental HCMV RVAD169. These
results demonstrate that the UL72 gene product is not a dUTPase and is not essential for replication in human fibroblasts.
Tipologia CRIS:
03A-Articolo su Rivista
Elenco autori:
CAPOSIO P; RIERA L; HAHN G; LANDOLFO S.; GRIBAUDO G
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