Nanobody-based immunomagnetic separation platform for rapid isolation and detection of Salmonella enteritidis in food samples
Articolo
Data di Pubblicazione:
2023
Abstract:
Rapid separation and identification of Salmonella enteritidis (S. enteritidis) in food is of great importance to prevent outbreaks of foodborne diseases. Herein, by using O and H antigens as targets, an epitope-based bio-panning strategy was applied to isolate specific nanobodies towards S. enteritidis. This method constitutes an efficient way to obtain specific antibody fragments and test pairwise nanobodies. On this basis, a double nanobody-based sandwich enzyme-linked immunosorbent assay (ELISA) coupled with immunomagnetic sepa-ration (IMS) was developed to rapid enrich and detect S. enteritidis in food. The detection limit of the IMS-ELISA was 3.2 x 103 CFU/mL. In addition, 1 CFU of S. enteritidis in food samples can be detected after 4-h cultivation, which was shortened by 2 h after IMS. The IMS-ELISA strategy could avoid matrix interference and shorten the enrichment culture time, which has great potential for application in monitoring bacterial contamination in food.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Single domain antibody; Salmonella; ELISA; Immunomagnetic separation; Rapid detection
Elenco autori:
Bai M.; Wang Y.; Zhang C.; Wang Y.; Wei J.; Liao X.; Wang J.; Anfossi L.; Wang Y.
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