Data di Pubblicazione:
2009
Abstract:
The European hazelnut (Corylus avellana L.) is a monoeciuos tree and exhibits sporophytic self-incompatibility. Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals. Self-incompatibility is controlled by a single multi-allelic locus, the S locus.
The involved molecular mechanisms are still partly unknown but in the Brassicaceae. Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut.
The Differential Display technique was applied for the study of the female determinant of self-incompatibility. Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom. The results allowed to isolate partial sequences that showed an interesting homology degree with transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the efforts for getting differentially expressed sequences of this type were increased. Primers were designed on conserved regions of serine-threonine kinase receptors. Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins. The isolated sequences are being studied to check their expression in different tissues and style developmental stages.
Tipologia CRIS:
04B-Conference paper in rivista
Keywords:
Corylus avellana L.; S locus; female determinant; Differential Display
Elenco autori:
Torello Marinoni D.; Beltramo C.; Akkak A.; Destefanis M.L.; Boccacci P.; R. Botta
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