Skip to Main Content (Press Enter)

Logo UNITO
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione

UNI-FIND
Logo UNITO

|

UNI-FIND

unito.it
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione
  1. Pubblicazioni

Variable membrane protein A of flavescence dorée phytoplasma binds the midgut perimicrovillar membrane of Euscelidius variegatus and promotes adhesion to its epithelial cells

Articolo
Data di Pubblicazione:
2018
Abstract:
Phytoplasmas are uncultivated plant pathogens and cell wall-less bacteria and are transmitted from plant to plant by hemipteran insects. The phytoplasma’s circulative propagative cycle in insects requires the crossing of the midgut and salivary glands, and primary adhesion to cells is an initial step toward the invasion process. The flavescence dorée (FD) phytoplasma possesses a set of variable membrane proteins (Vmps) exposed on its surface, and this pathogen is suspected to interact with insect cells. The results showed that VmpA is expressed by the flavescence dorée phytoplasma present in the midgut and salivary glands. Phytoplasmas cannot be cultivated at present, and no mutant can be produced to investigate the putative role of Vmps in the adhesion of phytoplasma to insect cells. To overcome this difficulty, we engineered the Spiroplasma citri mutant G/6, which lacks the ScARP adhesins, for VmpA expression and used VmpA-coated fluorescent beads to determine if VmpA acts as an adhesin in ex vivo adhesion assays and in vivo ingestion assays. VmpA specifically interacted with Euscelidius variegatus insect cells in culture and promoted the retention of VmpA-coated beads to the midgut of E. variegatus. In this latest case, VmpA-coated fluorescent beads were localized and embedded in the perimicrovillar membrane of the insect midgut. Thus, VmpA functions as an adhesin that could be essential in the colonization of the insect by the FD phytoplasmas.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
phytoplasma, spiroplasma, bacterial adhesion to cell, insect vector, transmission
Elenco autori:
Arricau-Bouvery, N., Duret, S., Dubrana, M. P., Batailler, B., Desqué, D., Béven, L., Danet J.L., Monticone M., Bosco, D, Malembic-Maher S. and Foissac, X.
Autori di Ateneo:
BOSCO Domenico
Link alla scheda completa:
https://iris.unito.it/handle/2318/1668948
Link al Full Text:
https://iris.unito.it/retrieve/handle/2318/1668948/415626/AEM02487-17R1_Merged_PDF-4.pdf
Pubblicato in:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Journal
  • Dati Generali

Dati Generali

URL

http://aem.asm.org/content/84/8/e02487-17.full.pdf+html
  • Utilizzo dei cookie

Realizzato con VIVO | Designed by Cineca | 25.4.2.0