Data di Pubblicazione:
2018
Abstract:
Purpose: The most significant prognostic factor in early breast cancer is lymph node involvement. This stage between localized and systemic disease is key to understanding breast cancer progression; however, our knowledge of the evolution of lymph node malignant invasion remains limited, as most currently available data are derived from primary tumors. Experimental Design: In 11 patients with treatment-na€ve node-positive early breast cancer without clinical evidence of distant metastasis, we investigated lymph node evolution using spatial multiregion sequencing (n ¼ 78 samples) of primary and lymph node deposits and genomic profiling of matched longitudinal circulating tumor DNA (ctDNA). Results: Linear evolution from primary to lymph node was rare (1/11), whereas the majority of cases displayed either early divergence between primary and nodes (4/11) or no detectable divergence (6/11), where both primary and nodal cells belonged to a single recent expansion of a metastatic clone. Divergence of metastatic subclones was driven in part by APOBEC. Longitudinal ctDNA samples from 2 of 7 subjects with evaluable plasma taken perioperatively reflected the two major evolutionary patterns and demonstrate that private mutations can be detected even from early metastatic nodal deposits. Moreover, node removal resulted in disappearance of private lymph node mutations in ctDNA. Conclusions: This study sheds new light on a crucial evolutionary step in the natural history of breast cancer, demonstrating early establishment of axillary lymph node metastasis in a substantial proportion of patients.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Adult; Aged; Aged, 80 and over; Axilla; Breast Neoplasms; Circulating Tumor DNA; Clonal Evolution; Female; Gene Expression Regulation, Neoplastic; Humans; Lymph Nodes; Lymphatic Metastasis; Middle Aged; Neoplasm Proteins; Neoplasm Staging
Elenco autori:
Barry P.; Vatsiou A.; Spiteri I.; Nichol D.; Cresswell G.D.; Acar A.; Trahearn N.; Hrebien S.; Garcia-Murillas I.; Chkhaidze K.; Ermini L.; Huntingford I.S.; Cottom H.; Zabaglo L.; Koelble K.; Khalique S.; Rusby J.E.; Muscara Francesca; Dowsett M.; Maley C.C.; Natrajan R.; Yuan Y.; Schiavon G.; Turner N.; Sottoriva A.
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