Skip to Main Content (Press Enter)

Logo UNITO
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione

UNI-FIND
Logo UNITO

|

UNI-FIND

unito.it
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione
  1. Pubblicazioni

Effect of the rearing diet on gene expression of antimicrobial peptides in Hermetia illucens (Diptera: Stratiomyidae)

Articolo
Data di Pubblicazione:
2023
Abstract:
Insect proteins have been proposed for human and animal food production. Safeguarding the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to entomopathogens. Therefore, new strategies for preserving insect health must be implemented. Modulation of the insect immune system through the diet is one such strategy. We evaluated gene expression of two antimicrobial peptides (one defensin and one cecropin) in Hermetia illucens (L.) (Diptera: Stratiomyidae) reared on different diets. Analyses were performed on prepupae and 10-day-old larvae reared on cereal- and municipal organic waste-based diets and on only prepupae reared on a cereal-based diet supplemented with sunflower, corn, or soybean oil. The inclusion of sunflower oil at different points in the cereal-based diet was also evaluated. Moreover, diet-driven differences in the inhibitory activity of the hemolymph were tested against Escherichia coli DH5 alpha and Micrococcus yunnanensis HI55 using diffusion assays in solid media. Results showed that a municipal organic waste-based diet produced a significant overexpression of antimicrobial peptides only in prepupae. Inclusion of vegetable oils caused an upregulation of at least one peptide, except for the corn oil. Higher expression of both genes was observed when sunflower oil was added 5 days before pupation. All hemolymph samples showed an inhibitory activity against bacteria colonies. Our results suggest that municipal organic waste-based diet and vegetable oil-added diet may successfully impact the immune system of H. illucens. Such alternatives may also exist for other species of economic interest.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
cecropin; cereal; defensin; hemolymph; organic municipal solid waste; vegetable oil
Elenco autori:
Candian, Valentina; Savio, Carlotta; Meneguz, Marco; Gasco, Laura; Tedeschi, Rosemarie
Autori di Ateneo:
CANDIAN VALENTINA
GASCO Laura
TEDESCHI Rosemarie
Link alla scheda completa:
https://iris.unito.it/handle/2318/1945448
Link al Full Text:
https://iris.unito.it/retrieve/handle/2318/1945448/1213690/Insect%20Science%20-%202022%20-%20Candian%20-%20Effect%20of%20the%20rearing%20diet%20on%20gene%20expression%20of%20antimicrobial%20peptides%20in%20Hermetia.pdf
Pubblicato in:
INSECT SCIENCE
Journal
Progetto:
Prog. "Economia circolare biomasse - nuovi prodotti: la bioconversione sostenibile degli insetti (BioSIn)"
  • Aree Di Ricerca

Aree Di Ricerca

Settori (12)


LS9_10 - Veterinary and applied animal sciences - (2022)

CIBO, AGRICOLTURA e ALLEVAMENTI - Agricoltura e Produzioni Vegetali

CIBO, AGRICOLTURA e ALLEVAMENTI - Allevamento e Produzioni Animali

CIBO, AGRICOLTURA e ALLEVAMENTI - Chimica e cibo

CIBO, AGRICOLTURA e ALLEVAMENTI - Farmacologia Veterinaria

CIBO, AGRICOLTURA e ALLEVAMENTI - Miglioramento e difesa delle colture

CIBO, AGRICOLTURA e ALLEVAMENTI - Tecnologie alimentari e microbiologia degli alimenti

MEDICINA, SALUTE e BENESSERE - Ricerca Traslazionale e Clinica

PIANETA TERRA, AMBIENTE, CLIMA, ENERGIA e SOSTENIBILITA' - Energia e Fonti Energetiche

PIANETA TERRA, AMBIENTE, CLIMA, ENERGIA e SOSTENIBILITA' - Foreste e Legno

PIANETA TERRA, AMBIENTE, CLIMA, ENERGIA e SOSTENIBILITA' - Risorsa suolo e ambiente

SCIENZE DELLA VITA e FARMACOLOGIA - Interazioni tra molecole, cellule, organismi e ambiente
  • Utilizzo dei cookie

Realizzato con VIVO | Designed by Cineca | 25.6.1.0