Skip to Main Content (Press Enter)

Logo UNITO
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione

UNI-FIND
Logo UNITO

|

UNI-FIND

unito.it
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione
  1. Pubblicazioni

Impact of active lifestyle on the primary school children saliva microbiota composition

Articolo
Data di Pubblicazione:
2023
Abstract:
The aim of the study was to evaluate the effects of Active or Sedentary lifestyle on saliva microbiota composition in Italian schoolchildren. Methods: Male (114) and female children (8-10 years) belonging to five primary schools in the neighborhoods of Turin were classified as active (A) or sedentary (S) based on PAQ-C-It questionnaire. PCR amplification of salivary DNA targeted the hypervariable V3-V4 regions of the 16S rRNA bacterial genes. DADA2 workflow was used to infer the Amplicon Sequence Variants and the taxonomic assignments; the beta-diversity was obtained by PCoA with the UniFrac method; LEfSe algorithm, threshold at 5%, and Log LDA cutoff at ±0.5 were used to identify differently abundant species in A compared to S saliva sample. Daily food intake was assessed by 3-Days food record. The metabolic potential of microbial communities was assessed by PICRUSt. Results: No significant differences were found in individual's gender distribution (p = 0.411), anthropometry, BMI (p > 0.05), and all diet composition between A and S groups (p > 0.05). Eight species were differently abundant: Prevotella nigrescens (LDA score = -3.76; FDR = 1.5×10-03), Collinsella aerofaciens (LDA score = -3.17; FDR = 7.45×10-03), Simonsiella muelleri (LDA score = -2.96; FDR = 2.76×10-05), Parabacteroides merdae (LDA score = -2.43; FDR = 1.3×10-02) are enriched in the A group; Gemella parahaemolysans, Prevotella aurantiaca (LDA score = -3.9; FDR = 5.27×10-04), Prevotella pallens (LDA score = 4.23; FDR = 1.93×10-02), Neisseria mucosa (LDA score = 4.43; FDR = 1.31×10-02; LDA score = 2.94; FDR = 7.45×10-03) are enriched in the S group. A prevalence of superpathway of fatty acid biosynthesis initiation (E. coli) and catechol degradation II (meta-cleavage pathway) was found in saliva from A compared to S children. Conclusion: Our results showed that active children had an enrichment of species and genera mainly associated with a healthier profile. By contrast, the genera and the species enriched in the sedentary group could be linked to human diseases.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
children; exercise; lifestyle; microbiota; saliva
Elenco autori:
Mancini, Annamaria; Cerulli, Claudia; Vitucci, Daniela; Lasorsa, Vito Alessandro; Parente, Daniela; Di Credico, Andrea; Orrù, Stefania; Brustio, Paolo Riccardo; Lupo, Corrado; Rainoldi, Alberto; Schena, Federico; Capasso, Mario; Buono, Pasqualina
Autori di Ateneo:
BRUSTIO Paolo Riccardo
LUPO Corrado
RAINOLDI Alberto
Link alla scheda completa:
https://iris.unito.it/handle/2318/1933093
Link al Full Text:
https://iris.unito.it/retrieve/handle/2318/1933093/1190534/Mancini%20et%20al.%20(2023)%20Impact%20of%20active%20lifestyle%20on%20the%20primary%20school%20children%20saliva%20microbiota%20composition.pdf
Pubblicato in:
FRONTIERS IN NUTRITION
Journal
Progetto:
Programmi di Rilevante Interesse Nazionale - Bando PRIN 2017
  • Aree Di Ricerca

Aree Di Ricerca

Settori (10)


LS4_7 - Nutrition and exercise physiology - (2022)

CIBO, AGRICOLTURA e ALLEVAMENTI - Farmacologia Veterinaria

CIBO, AGRICOLTURA e ALLEVAMENTI - Patologia e malattie degli animali

CIBO, AGRICOLTURA e ALLEVAMENTI - Scienze cliniche veterinarie

MEDICINA, SALUTE e BENESSERE - Epidemiologia

MEDICINA, SALUTE e BENESSERE - Oncologia e Tumori

MEDICINA, SALUTE e BENESSERE - Ricerca Traslazionale e Clinica

MEDICINA, SALUTE e BENESSERE - Trapianti e medicina rigenerativa

SCIENZE DELLA VITA e FARMACOLOGIA - Basi molecolari e cellulari delle patologie

SCIENZE DELLA VITA e FARMACOLOGIA - Interazioni tra molecole, cellule, organismi e ambiente
  • Utilizzo dei cookie

Realizzato con VIVO | Designed by Cineca | 25.6.1.0