Skip to Main Content (Press Enter)

Logo UNITO
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione

UNI-FIND
Logo UNITO

|

UNI-FIND

unito.it
  • ×
  • Home
  • Pubblicazioni
  • Progetti
  • Persone
  • Competenze
  • Settori
  • Strutture
  • Terza Missione
  1. Pubblicazioni

Improving the sensitivity and the cost-effectiveness of a competitive visual lateral flow immunoassay through sequential designs of experiments

Articolo
Data di Pubblicazione:
2025
Abstract:
Although visual lateral flow immunoassays (LFIAs) are advantageous analytical tools, they typically suffer from low sensitivity in the competitive format (cLFIA). Optimizing sensitivity in this format is challenging as it depends on the inhibition of a visual signal. To optimize the sensitivity of a cLFIA, an experimental design-based approach is proposed and illustrated for the detection of cortisol. Gold nanoparticles (AuNPs) are used to label monoclonal anti-cortisol antibodies (Ab) to generate the probe. The Ab:AuNP ratio, the amount of probe and competitor on the test line are proposed as relevant parameters for optimization. As discriminatory conditions for sensitivity, the simultaneous disappearance of the color in the presence of a certain level of cortisol and a clearly visible color in its absence were assumed. A decision process called 4S (start, shift, sharpen, stop) is proposed for the interpretation of the response surfaces obtained after multivariate modelling of the data set. Using this approach, sensitivity was improved > 500-fold (visual LOD = 20.5 +/- 1.0 ng/mL, LOD = 0.28 ng/mL) after 13 experiments and 10-fold (vLOD = 2.2 +/- 0.1 ng/mL, LOD = 0.07 ng/mL) after 21 additional experiments. Reagent consumption was simultaneously reduced 40-fold. The feasibility of cortisol detection in human saliva samples was established with a satisfactory recovery of 70-109 %. The strategy from this work is useful for designing highly sensitive cLFIAs by unravelling the full potential of the immunoreagents and labels employed, with a limited number of experiments and reagent consumption, and without sacrificing the simplicity and affordability of visual, stand-alone LFIAs.
Tipologia CRIS:
03A-Articolo su Rivista
Keywords:
Point-of-care testing; Rapid test; Immunosensor; Gold Nanoparticles; Cortisol; Immunochromatographic assay; Visual LOD
Elenco autori:
Cavalera, Simone; Gelli, Alessandro; Di Nardo, Fabio; Serra, Thea; Testa, Valentina; Bertinetti, Stefano; Ozella, Laura; Forte, Claudio; Baggiani, Claudio; Anfossi, Laura
Autori di Ateneo:
ANFOSSI Laura
BAGGIANI Claudio
BERTINETTI Stefano
CAVALERA Simone
DI NARDO Fabio
FORTE Claudio
OZELLA Laura
SERRA THEA
TESTA VALENTINA
Link alla scheda completa:
https://iris.unito.it/handle/2318/2054330
Link al Full Text:
https://iris.unito.it/retrieve/handle/2318/2054330/1531479/mcj25_208_112450_draft.pdf
Pubblicato in:
MICROCHEMICAL JOURNAL
Journal
  • Aree Di Ricerca

Aree Di Ricerca

Settori (5)


PE4_5 - Analytical chemistry - (2024)

PIANETA TERRA, AMBIENTE, CLIMA, ENERGIA e SOSTENIBILITA' - Energia e Fonti Energetiche

SCIENZE DELLA VITA e FARMACOLOGIA - Chimica Analitica e Farmaceutica

SCIENZE DELLA VITA e FARMACOLOGIA - Tecnologie Farmaceutiche e Cosmetiche

SCIENZE MATEMATICHE, CHIMICHE, FISICHE - Materiali Avanzati
  • Utilizzo dei cookie

Realizzato con VIVO | Designed by Cineca | 25.6.1.0