Data di Pubblicazione:
2011
Abstract:
A Real-Time PCR Allelic Discrimination TaqMan assay based on the analysis of one diagnosis position
enabling the identification of cows’ and buffalo milk in dairy products was developed. Specific primers
and probes were designed on the mitochondrial cytochrome b gene. In particular, primers were designed
upstream and downstream the chosen diagnosis site in a well conserved region for both Bos taurus and
Bubalus bubalis. Two probes were designed to specifically hybridise to B. taurus and B. bubalis sequences.
This technique proved to be species-specific when tested on blood and pure milk samples giving an
unambiguous result plot of rapid interpretation. The detection threshold, evaluated by means of different
dilutions, was 2% of cow milk in buffalo milk.
The test, when applied to cheese samples from the retail trade, enabled the identification of partial or
total substitution of cow for buffalo milk. Furthermore, it is relatively rapid, as both species can be
detected in one step, with no need for any post-PCR manipulation.
Tipologia CRIS:
03A-Articolo su Rivista
Elenco autori:
A. Dalmasso; T. Civera; F. La Neve; M.T. Bottero
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